ARG62347

antibody from Arigo

Targeting: TUBB1 dJ543J19.4

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

ARG62347

Provider

Arigo

Product name

anti-beta Tubulin antibody [BT7R]

Antibody type

Monoclonal

Antigen

β-Tubulin N-terminal peptide-KLH conjugates.

Reactivity

Human, Mouse, Rat, Chicken/Avian, Rabbit, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

BT7R

Vial size

100 µg

Concentration

1 mg/ml

Storage

Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 1) U87-MG 2) SW480 stained with ARG62347 anti-beta Tubulin antibody [BT7R] at 1:1000 dilution. Data provided by customer.

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western Blot: 10 µg mouse brain tissue lysates at 1: 2000 dilution stained with beta Tubulin antibody (ARG62605). 50kDa band is beta Tubulin, detected by beta Tubulin antibody [BT7R] (ARG62347) at 1: 2000 dilution (0.5 µg/mL); 42kDa band is beta Actin, detected by beta Actin antibody [BA3R] (ARG62346) at 1: 1000 dilution (1 µg/mL); 37kDa band is GAPDH, detected by GAPDH antibody [GA1R] at 1: 5000 dilution (0.2 µg/mL).

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western Blot: mouse brain tissue lysates at 1: 4000 dilution stained with beta Tubulin antibody [BT7R] (ARG62347).