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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NBP2-52577 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal beta I Tubulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta I Tubulin Antibody (2A1A9) [NBP2-52577] - Analysis using TUBB1 mAb against HEK293 (1) and TUBB1 (AA: 33-166)-hIgGFc transfected HEK293 (2) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta I Tubulin Antibody (2A1A9) [NBP2-52577] - Analysis using TUBB1 mAb against human TUBB1 (AA: 33-166) recombinant protein. (Expected MW is 40.5 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta I Tubulin Antibody (2A1A9) [NBP2-52577] - Analysis using beta I Tubulin mouse mAb against K562 (1), HepG2 (2), A431 (3), Jurkat (4), Hela (5), NIH/3T3 (6), Cos7 (7) and PC12 (8) cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: beta I Tubulin Antibody (2A1A9) [NBP2-52577] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: beta I Tubulin Antibody (2A1A9) [NBP2-52577] - Analysis of paraffin-embedded ovarian cancer tissues using TUBB1 mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: beta I Tubulin Antibody (2A1A9) [NBP2-52577] - Analysis of paraffin-embedded ovarian cancer tissues using TUBB1 mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: beta I Tubulin Antibody (2A1A9) [NBP2-52577] - Analysis of A431 cells using TUBB1 mouse mAb (green) and negative control (red).
