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- References [14]
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- Product number
- A05397-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Tubulin beta Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Tubulin beta detection. Tested with WB, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Elevated retinal retinol-binding protein 4 levels in diabetic mice can induce retinal neurodegeneration through microglia.
Fufang Zhenzhu Tiaozhi Capsule Prevents Intestinal Inflammation and Barrier Disruption in Mice With Non-Alcoholic Steatohepatitis.
Circular RNA screening identifies circMYLK4 as a regulator of fast/slow myofibers in porcine skeletal muscles.
ELOVL2 restrains cell proliferation, migration, and invasion of prostate cancer via regulation of the tumor suppressor INPP4B.
What Happened in the Hippocampal Axon in a Rat Model of Posttraumatic Stress Disorder.
The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway.
TSPO deficiency accelerates amyloid pathology and neuroinflammation by impairing microglial phagocytosis.
Antiproliferative activity of berberine in HepG2 cells via inducing apoptosis and arresting cell cycle.
Ferulic acid alleviates lipotoxicity-induced hepatocellular death through the SIRT1-regulated autophagy pathway and independently of AMPK and Akt in AML-12 hepatocytes.
Panax notoginseng Saponins Regulate Macrophage Polarization under Hyperglycemic Condition via NF-κB Signaling Pathway.
Inhibition of neurite outgrowth using commercial myelin associated glycoprotein-Fc in neuro-2a cells.
Ibrutinib targets microRNA-21 in multiple myeloma cells by inhibiting NF-κB and STAT3.
Phosphatidylinositol 3-Kinase/Akt signal pathway resists the apoptosis and inflammation in human extravillous trophoblasts induced by Porphyromonas gingivalis.
NEAT1 upregulates EGCG-induced CTR1 to enhance cisplatin sensitivity in lung cancer cells.
Xu Z, Bai S, Wu H, Fang M
Microscopy research and technique 2023 Feb;86(2):223-231
Microscopy research and technique 2023 Feb;86(2):223-231
Fufang Zhenzhu Tiaozhi Capsule Prevents Intestinal Inflammation and Barrier Disruption in Mice With Non-Alcoholic Steatohepatitis.
Lan T, Xu T, Fu Y, Jiang S, Liang X, Yu Z, Pan L, Rong X, Guo J
Frontiers in endocrinology 2022;13:864703
Frontiers in endocrinology 2022;13:864703
Circular RNA screening identifies circMYLK4 as a regulator of fast/slow myofibers in porcine skeletal muscles.
Cao H, Liu J, Du T, Liu Y, Zhang X, Guo Y, Wang J, Zhou X, Li X, Yang G, Shi X
Molecular genetics and genomics : MGG 2022 Jan;297(1):87-99
Molecular genetics and genomics : MGG 2022 Jan;297(1):87-99
ELOVL2 restrains cell proliferation, migration, and invasion of prostate cancer via regulation of the tumor suppressor INPP4B.
Hu T, Zhang H, Du Y, Luo S, Yang X, Zhang H, Feng J, Chen X, Tu X, Wang C, Zhang Y
Cellular signalling 2022 Aug;96:110373
Cellular signalling 2022 Aug;96:110373
What Happened in the Hippocampal Axon in a Rat Model of Posttraumatic Stress Disorder.
Guan Y, Chen X, Zhao B, Shi Y, Han F
Cellular and molecular neurobiology 2022 Apr;42(3):723-737
Cellular and molecular neurobiology 2022 Apr;42(3):723-737
The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway.
Zhang Q, Li J, Zhong H, Xu Y
Molecular medicine (Cambridge, Mass.) 2021 Sep 20;27(1):115
Molecular medicine (Cambridge, Mass.) 2021 Sep 20;27(1):115
TSPO deficiency accelerates amyloid pathology and neuroinflammation by impairing microglial phagocytosis.
Zhang H, Wang H, Gao F, Yang J, Xu Y, Fu Y, Cai M, Zhang X, Yang Q, Tong K, Hu Y, Chen H, Ma C, He W, Zhang J
Neurobiology of aging 2021 Oct;106:292-303
Neurobiology of aging 2021 Oct;106:292-303
Antiproliferative activity of berberine in HepG2 cells via inducing apoptosis and arresting cell cycle.
Yang L, Cao J, Wei J, Deng J, Hou X, Hao E, Du Z, Zou L, Li P
Food & function 2021 Nov 29;12(23):12115-12126
Food & function 2021 Nov 29;12(23):12115-12126
Ferulic acid alleviates lipotoxicity-induced hepatocellular death through the SIRT1-regulated autophagy pathway and independently of AMPK and Akt in AML-12 hepatocytes.
Xu T, Song Q, Zhou L, Yang W, Wu X, Qian Q, Chai H, Han Q, Pan H, Dou X, Li S
Nutrition & metabolism 2021 Jan 19;18(1):13
Nutrition & metabolism 2021 Jan 19;18(1):13
Panax notoginseng Saponins Regulate Macrophage Polarization under Hyperglycemic Condition via NF-κB Signaling Pathway.
Zhao Y, Zheng J, Yu Y, Wang L
BioMed research international 2018;2018:9239354
BioMed research international 2018;2018:9239354
Inhibition of neurite outgrowth using commercial myelin associated glycoprotein-Fc in neuro-2a cells.
Liu F, Gao ML, Bai J, Wang YF, Li XQ
Neural regeneration research 2018 Nov;13(11):1893-1899
Neural regeneration research 2018 Nov;13(11):1893-1899
Ibrutinib targets microRNA-21 in multiple myeloma cells by inhibiting NF-κB and STAT3.
Ma J, Gong W, Liu S, Li Q, Guo M, Wang J, Wang S, Chen N, Wang Y, Liu Q, Zhao H
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2018 Jan;40(1):1010428317731369
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2018 Jan;40(1):1010428317731369
Phosphatidylinositol 3-Kinase/Akt signal pathway resists the apoptosis and inflammation in human extravillous trophoblasts induced by Porphyromonas gingivalis.
Guo H, Ren H, Liang S, Ji Y, Jiang H, Zhang P, Du M
Molecular immunology 2018 Dec;104:100-107
Molecular immunology 2018 Dec;104:100-107
NEAT1 upregulates EGCG-induced CTR1 to enhance cisplatin sensitivity in lung cancer cells.
Jiang P, Wu X, Wang X, Huang W, Feng Q
Oncotarget 2016 Jul 12;7(28):43337-43351
Oncotarget 2016 Jul 12;7(28):43337-43351
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Tubulin beta using anti-Tubulin beta antibody (A05397-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tubulin beta antigen affinity purified polyclonal antibody (Catalog # A05397-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tubulin beta at approximately 50KD. The expected band size for Tubulin beta is at 50KD.
- Additional image
