Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX83725 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83725, RRID:AB_10726861
- Product name
- RANGAP1 antibody [1B4]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RANGAP1 monoclonal antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RANGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RANGAP1.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-RANGAP1 mouse monoclonal antibody (GTX83725) immunofluorescent staining of COS7 cells transiently transfected with RANGAP1
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-RANGAP1 mouse monoclonal antibody. (GTX83725, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RANGAP1 mouse monoclonal antibody. (GTX83725, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-RANGAP1 mouse monoclonal antibody. (GTX83725, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-RANGAP1 antibody(GTX83725),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-RANGAP1 antibody(GTX83725),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC204223 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RANGAP1 antibody(GTX83725), and then analyzed by flow cytometry.
