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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PAB27659 - Provider product page
- Provider
- Abnova Corporation
- Product name
- AP2A1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of AP2A1.
- Antigen sequence
ELEPPAPESPMALLADPAPAAD- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- AP2A1 polycolnal antibody (Cat # PAB27659) (0.01ug/ml) staining of human frontal cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- AP2A1 polycolnal antibody (Cat # PAB27659) (0.1ug/ml) staining of mouse brain lysate (~5ug protein in SDSPAGE buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- AP2A1 polycolnal antibody (Cat # PAB27659) (0.1ug/ml) staining of methanol-fixed HeLa cells with stably expressing Mouse Ap2a1. Primary incubation was 1 hour. Detected by Alexa Fluor 594. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK.
- Validation comment
- Immunocytochemistry
