- 03-4400 - Invitrogen Antibodies ANXA2 antibody

Submitted references Annexin A2 Enhances Complement Activation by Inhibiting Factor H.

Renner B, Tong HH, Laskowski J, Jonscher K, Goetz L, Woolaver R, Hannan J, Li YX, Hourcade D, Pickering MC, Holers VM, Thurman JM
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1355-65

Up-regulation of annexin A2 in cholangiocarcinoma caused by Opisthorchis viverrini and its implication as a prognostic marker.

Yonglitthipagon P, Pairojkul C, Chamgramol Y, Mulvenna J, Sripa B
International journal for parasitology 2010 Aug 15;40(10):1203-12

Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells.

Hastie C, Masters JR, Moss SE, Naaby-Hansen S
The Journal of biological chemistry 2008 May 2;283(18):12595-603

Involvement of annexin A2 in anti-beta2GPI/beta2GPI-induced tissue factor expression on monocytes.

Zhou H, Ling S, Yu Y, Wang T, Hu H
Cell research 2007 Aug;17(8):737-9

An annexin 2 phosphorylation switch mediates p11-dependent translocation of annexin 2 to the cell surface.

Deora AB, Kreitzer G, Jacovina AT, Hajjar KA
The Journal of biological chemistry 2004 Oct 15;279(42):43411-8

Annexin II mediates plasminogen-dependent matrix invasion by human monocytes: enhanced expression by macrophages.

Brownstein C, Deora AB, Jacovina AT, Weintraub R, Gertler M, Khan KM, Falcone DJ, Hajjar KA
Blood 2004 Jan 1;103(1):317-24

Integrin alphaMbeta2 orchestrates and accelerates plasminogen activation and fibrinolysis by neutrophils.

Pluskota E, Soloviev DA, Bdeir K, Cines DB, Plow EF
The Journal of biological chemistry 2004 Apr 23;279(17):18063-72

Inactivation of annexin II tetramer by S-nitrosoglutathione.

Liu L, Enright E, Sun P, Tsai SY, Mehta P, Beckman DL, Terrian DM
European journal of biochemistry 2002 Sep;269(17):4277-86

Suppressive effect on lymphoproliferation in vitro by soluble annexin II released from isolated placental membranes.

Aarli A, Kristoffersen EK, Jensen TS, Ulvestad E, Matre R
American journal of reproductive immunology (New York, N.Y. : 1989) 1997 Nov;38(5):313-9

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of WI-38 cell lysates using Annexin II monoclonal antibody, mouse (clone Z014) (Product # 03-4400)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of WI-38 cell lysates using Annexin II monoclonal antibody, mouse (clone Z014) (Product # 03-4400)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Annexin A2 was achieved by transfecting HeLa with Annexin A2 specific siRNAs (Silencer® select Product # S1384, S1385). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Annexin A2 untransfected cells (lane 1), non-targeting scrambled siRNA transfected cells (lane 2) and knockdown cells (lane 3). The blot was probed with Annexin A2 Monoclonal Antibody (Z014) (Product # 03-4400, 1:1000 dilution ) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Annexin A2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Annexin A2 Monoclonal Antibody (Z014)(Product # 03-4400) and a 38kDa band corresponding to Annexin A2 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of A549 (Lane 1), HCT 116 (Lane 2), HeLa (Lane 3), Mouse Testis (Lane 4), Rat Testis (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Annexin A2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 15 minutes at room temperature. The cells were labeled with Annexin A2 Monoclonal Antibody (Z014) (Product # 03-4400) at 5 µg in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.