Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- MA5-32689 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Annexin A2 Recombinant Rabbit Monoclonal Antibody (JA42-30)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JA42-30
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Annexin A2 in different lysates using a Monoclonal antibody (Product #MA5-32689) at a dilution of 1:500. Positive control: Lane 1: Hela Lane 2: Human kidney Lane 3: Murine testis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Annexin A2 in different lysates using a Monoclonal antibody (Product #MA5-32689) at a dilution of 1:500. Positive control: Lane 1: Hela Lane 2: Human kidney Lane 3: Murine testis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Annexin A2 was achieved by transfecting Hep G2 with Annexin A2 specific siRNAs (Silencer® select Product # S1384, S1385). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Hep G2 untransfected cells (lane 1), non-targeting scrambled siRNA transfected cells (lane 2) and Annexin A2 knockdown cells (lane 3). The blot was probed with Annexin A2 Recombinant Rabbit Monoclonal Antibody (JA42-30) (Product # MA5-32689, 1:1000 dilution ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Annexin A2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Annexin A2 Recombinant Rabbit Monoclonal Antibody (JA42-30)(Product # MA5-32689) and a 38kDa band corresponding to Annexin A2 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of A549 (Lane 1), HCT 116 (Lane 2), HeLa (Lane 3), Mouse Testis (Lane 4), Rat Testis (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Annexin A2 in Hela cells using a Annexin A2 Monoclonal antibody (Product # MA5-32689) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Annexin A2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Annexin A2 Recombinant Rabbit Monoclonal Antibody (JA42-30) (Product # MA5-32689) at 5 µg in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing Cytoplasm localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Annexin A2 of paraffin-embedded Human liver tissue using a Annexin-A2 Monoclonal antibody (Product #MA5-32689). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Annexin A2 of paraffin-embedded Human colon cancer tissue using a Annexin-A2 Monoclonal antibody (Product #MA5-32689). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Annexin A2 of paraffin-embedded Human prostate tissue using a Annexin-A2 Monoclonal antibody (Product #MA5-32689). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of Annexin A2 in K562 cells using a Annexin A2 Monoclonal Antibody (Product # MA5-32689) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).