60051-1-IG
antibody from Invitrogen Antibodies
Targeting: ANXA2
ANX2, ANX2L4, CAL1H, LIP2, LPC2D
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [3]
- Immunohistochemistry [6]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 60051-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Annexin A2 Monoclonal Antibody (3H1B11)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSTVHEILC KLSLEGDHST PPSAYGSVKA YTNFDAERDA LNIETAIKTK GVDEVTIVNI LTNRSNAQRQ DIAFAYQRRT KKELASALKS ALSGHLETVI LGLLKTPAQY DASELKASMK GLGTDEDSLI EIICSRTNQE LQEINRVYKE MYKTDLEKDI ISDTSGDFRK LMVALAKGRR AEDGSVIDYE LIDQDARDLY DAGVKRKGTD VPKWISIMTE RSVPHLQKVF DRYKSYSPYD MLESIRKEVK GDLENAFLNL VQCIQNKPLY FADRLYDSMK GKGTRDKVLI RIMVSRSEVD MLKIRSEFKR KYGKSLYYYI QQDTKGDYQK ALLYLCGGDD (1-339 aa encoded by BC016774)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3H1B11
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 60051-1-IG (ANXA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 60051-1-Ig (ANXA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of Annexin A2 antibody (60051-1-IG, 1:20,000) with si-Control and si-Annexin A2 transfected HEK293 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 60051-1-Ig (ANXA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using ANXA2 antibody 66051-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using ANXA2 antibody 60051-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 60051-1-IG (Annexin A2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas slide using 60051-1-Ig (ANXA2 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas slide using 60051-1-Ig (ANXA2 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60051-1-IG ( Annexin A2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60051-1-IG ( Annexin A2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug Annexin A2 antibody (60051-1-IG, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Annexin A2 (IP:60051-1-IG, 4ug; Detection:60051-1-IG 1:1500) with HeLa cells lysate 2400ug.