M00220-1

antibody from Boster Biological Technology

Targeting: CD86 B7-2, B7.2, CD28LG2

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00220-1

Provider

Boster Biological Technology

Product name

Anti-CD86/B7 2 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for B7 2/CD86 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Rat B7 2/CD86 information: Molecular Weight: 37682 MW; Subcellular Localization: Cell membrane; Single-pass type I membrane protein; Tissue Specificity: Expressed by activated B-lymphocytes and monocytes.

Reactivity

Human, Rat

Host

Rabbit

Antibody clone number

BFF-3

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human tonsil, using CD86 Antibody (M00220-1)CD86 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD86 Antibody (M00220-1)overnight at 4

Additional image