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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 942-RBM4-P0 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD86 Recombinant Rabbit Monoclonal Antibody (C86, 2160R)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- C86, 2160R
- Vial size
- 20 µg
- Concentration
- 200 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Ramos cell lysate using CD86 Recombinant Monoclonal Antibody (Product # 942-RBM4-P1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of Raji cells using CD86 Recombinant Monoclonal Antibody (Product # 942-RBM4-P1) followed by goat anti-rabbit IgG conjugated with CF488 (green). Nuclei are stained with Red Dot..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human colon using CD86 Recombinant Monoclonal Antibody (Product # 942-RBM4-P1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Ramos cells using CD86 Recombinant Monoclonal Antibody (Product # 942-RBM4-P1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SDS-PAGE Analyis Purified CD86 Recombinant Monoclonal Antibody (Product # 942-RBM4-P1). Confirmation of Purity and Integrity of Antibody.
