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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 305410 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#305410, RRID:AB_314530
- Product name
- LEAF™ Purified anti-human CD86 [IT2.2]
- Antibody type
- Monoclonal
- Antigen
- CD86
- Description
- CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 µg
- Concentration
- 1 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood monocytes were stained with LEAF™ purified CD86 (clone IT2.2) (filled histogram) or LEAF™ purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
- Conjugate
- Unconjugated
