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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB3238 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB3238, RRID:AB_10633970
- Product name
- Cd86 monoclonal antibody, clone GL1
- Antibody type
- Monoclonal
- Description
- Rat monoclonal antibody raised against Cd86.
- Isotype
- IgG
- Antibody clone number
- GL1
- Storage
- Store at 4°C.
Submitted references Distinct roles for the costimulatory ligands B7-1 and B7-2 in T helper cell differentiation?
Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers.
Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function.
Thompson CB
Cell 1995 Jun 30;81(7):979-82
Cell 1995 Jun 30;81(7):979-82
Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers.
Han S, Hathcock K, Zheng B, Kepler TB, Hodes R, Kelsoe G
Journal of immunology (Baltimore, Md. : 1950) 1995 Jul 15;155(2):556-67
Journal of immunology (Baltimore, Md. : 1950) 1995 Jul 15;155(2):556-67
Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function.
Hathcock KS, Laszlo G, Pucillo C, Linsley P, Hodes RJ
The Journal of experimental medicine 1994 Aug 1;180(2):631-40
The Journal of experimental medicine 1994 Aug 1;180(2):631-40
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- Abnova Corporation (provider)
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- Experimental details
- BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Cd86 monoclonal antibody, clone GL1 (Cat # MAB3238)-FITC. A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
- Validation comment
- Flow Cytometry
