PAB21367

antibody from Abnova Corporation

Targeting: SMARCC2 BAF170, CRACC2, Rsc8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21367

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21367, RRID:AB_10966513

Product name

SMARCC2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant SMARCC2.

Antigen sequence

AFGLESSGIAGTTSDEPERIEESGNDEARVEGQAT
DEKKEPKEPREGGGAIEEEAKEKTSEAPKKDEEKG
KEGDSEKESEKSDGDPIVDPEKEKEPKEGQEEVLK
EVVESEGERKTKVERDIGEGNLSTAA


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with SMARCC2 polyclonal antibody (Cat # PAB21367) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with SMARCC2 polyclonal antibody (Cat # PAB21367) at 1-4 ug/mL dilution shows positivity in nuclei but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach with SMARCC2 polyclonal antibody (Cat # PAB21367) shows strong nuclear positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)