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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 12018-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BAF170 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: KRSPSPSPT PEAKKKNAKK GPSTPYTKSK RGHREEEQED LTKDMDEPSP VPNVEEVTLP KTVNTKKDSE SAPVKGGTMT DLDEQEDESM ETTGKDEDEN STGNKGEQTK NPDLHEDNVT EQTHHIIIPS YAAWFDYNSV HAIERRALPE FFNGKNKSKT PEIYLAYRNF MIDTYRLNPQ EYLTSTACRR NLAGDVCAIM RVHAFLEQWG LINYQVDAES RPTPMGPPPT SHFHVLADTP SGLVPLQPKT PQGRQVDADT KAGRKGKELD DLVPETAKGK PELQTSASQQ MLNFPDKGKE KPTDMQNFGL RTDMYTKKNV PSKSKAAASA TREWTEQETL LLLEALEMY (300-647 aa encoded by BC013045)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.74 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using SMARCC2 antibody 12018-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human medulloblastoma using 12018-1-AP (SMARCC2 antibody) at dilution of 1:50 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-BAF170 (IP:12018-1-AP, 4ug; Detection:12018-1-AP 1:700) with HeLa cells lysate 3200ug.
