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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A301-039A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMARCC2/BAF170 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 0.20 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse SMARCC2/BAF170 by western blot. Samples: Whole cell lysate from HeLa (5, 15, 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-SMARCC2/BAF170 antibody (Product # A301-039A; lot A301-039A-1) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse SMARCC2/BAF170 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SMARCC2/BAF170 antibody A301-039A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. SMARCC2/BAF170 was also immunoprecipitated by rabbit anti-SMARCC2/BAF170 antibody A301-038A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMARCC2/BAF170 by immunohistochemistry. Sample: FFPE section of human skin basal cell carcinoma. Antibody: Affinity purified rabbit anti- SMARCC2/BAF170 (Cat. No. A301-039A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMARCC2/BAF170 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCC2/BAF170 antibody (Product # A301-039A; lot A301-039A-1) used for IP at 3 µg per reaction. SMARCC2/BAF170 was also immunoprecipitated by rabbit anti-SMARCC2/BAF170 antibody (Product # A301-038A). For blotting immunoprecipitated SMARCC2/BAF170 (Product # A301-039A) was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.
