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Learnsc-9972
antibody from Santa Cruz Biotechnology
Targeting: CDC27
ANAPC3, APC3, D0S1430E, D17S978E, NUC2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-9972 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-9972, RRID:AB_627228
- Product name
- Anti-CDC27
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Binding partner switching on microtubules and aurora-B in the mitosis to cytokinesis transition.
Regulation of late G1/S phase transition and APC Cdh1 by reactive oxygen species.
TheRae1 –Nup98 complex prevents aneuploidy by inhibiting securin degradation
Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27.
A conserved cyclin-binding domain determines functional interplay between anaphase-promoting complex-Cdh1 and cyclin A-Cdk2 during cell cycle progression.
Ozlü N, Monigatti F, Renard BY, Field CM, Steen H, Mitchison TJ, Steen JJ
Molecular & cellular proteomics : MCP 2010 Feb;9(2):336-50
Molecular & cellular proteomics : MCP 2010 Feb;9(2):336-50
Regulation of late G1/S phase transition and APC Cdh1 by reactive oxygen species.
Havens CG, Ho A, Yoshioka N, Dowdy SF
Molecular and cellular biology 2006 Jun;26(12):4701-11
Molecular and cellular biology 2006 Jun;26(12):4701-11
The
Karthik B. Jeganathan, Liviu Malureanu, Jan M. van Deursen
Nature 2005 Dec;438(7070):1036-1039
Nature 2005 Dec;438(7070):1036-1039
Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27.
Schwickart M, Havlis J, Habermann B, Bogdanova A, Camasses A, Oelschlaegel T, Shevchenko A, Zachariae W
Molecular and cellular biology 2004 Apr;24(8):3562-76
Molecular and cellular biology 2004 Apr;24(8):3562-76
A conserved cyclin-binding domain determines functional interplay between anaphase-promoting complex-Cdh1 and cyclin A-Cdk2 during cell cycle progression.
Sørensen CS, Lukas C, Kramer ER, Peters JM, Bartek J, Lukas J
Molecular and cellular biology 2001 Jun;21(11):3692-703
Molecular and cellular biology 2001 Jun;21(11):3692-703
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 92
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
