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- Antigen structure
- References [3]
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- Western blot [1]
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- Product number
- PAB9988 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9988, RRID:AB_1676123
- Product name
- CDC27 (phospho T244) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC27.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Early mitotic degradation of the homeoprotein HOXC10 is potentially linked to cell cycle progression.
The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis.
Mad2 transiently associates with an APC/p55Cdc complex during mitosis.
Gabellini D, Colaluca IN, Vodermaier HC, Biamonti G, Giacca M, Falaschi A, Riva S, Peverali FA
The EMBO journal 2003 Jul 15;22(14):3715-24
The EMBO journal 2003 Jul 15;22(14):3715-24
The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis.
Topper LM, Campbell MS, Tugendreich S, Daum JR, Burke DJ, Hieter P, Gorbsky GJ
Cell cycle (Georgetown, Tex.) 2002 Jul-Aug;1(4):282-92
Cell cycle (Georgetown, Tex.) 2002 Jul-Aug;1(4):282-92
Mad2 transiently associates with an APC/p55Cdc complex during mitosis.
Wassmann K, Benezra R
Proceedings of the National Academy of Sciences of the United States of America 1998 Sep 15;95(19):11193-8
Proceedings of the National Academy of Sciences of the United States of America 1998 Sep 15;95(19):11193-8
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using CDC27 (phospho T244) polyclonal antibody (Cat # PAB9988) shows detection of a band ~92 KDa corresponding to phosphorylated human CDC27 (arrowhead).Lane 1 shows lysate from asynchronous cells.Lane 2 shows lysate from cells treated with nocodazole.Phosphorylated CDC27 is mostly present only in cell preparations arrested in mitosis.Each lane contains approximately 30 ug of HeLa whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose.After blocking with 5% non-fat dry milk in TTBS, the membrane was probed with the primary antibody diluted to 1 : 500 for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.ECL reagent was used for detection.Data contributed by Bing Li, UT South western.
