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Learn600-401-868
antibody from Rockland Immunochemicals, Inc.
Targeting: CDC27
ANAPC3, APC3, D0S1430E, D17S978E, NUC2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 600-401-868 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-868, RRID:AB_2075293
- Product name
- Anti-CDC27 (RABBIT) Antibody - 600-401-868
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Chronic low dose arsenic exposure preferentially perturbs mitotic phase of the cell cycle.
Ganapathy S, Liu J, Xiong R, Yu T, Makriyannis A, Chen C
Genes & cancer 2019 Feb;10(1-2):39-51
Genes & cancer 2019 Feb;10(1-2):39-51
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's Affinity Purified anti-cdc27 antibody shows detection of a band ~90 kDa corresponding to human cdc27 (arrowhead). Approximately 35 ug of HeLa whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0?ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
- Validation comment
- Western Blot
