sc-28366

antibody from Santa Cruz Biotechnology

Targeting: RAPGEF3 bcm910, cAMP-GEFI, EPAC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-28366

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-28366, RRID:AB_627521

Product name

Anti-RAPGEF3

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

References

Submitted references

Epac1 mediates protein kinase A-independent mechanism of forskolin-activated intestinal chloride secretion.

Hoque KM, Woodward OM, van Rossum DB, Zachos NC, Chen L, Leung GP, Guggino WB, Guggino SE, Tse CM
The Journal of general physiology 2010 Jan;135(1):43-58

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Exchange protein activated by cAMP (Epac) mediates cAMP-dependent but protein kinase A-insensitive modulation of vascular ATP-sensitive potassium channels.

Purves GI, Kamishima T, Davies LM, Quayle JM, Dart C
The Journal of physiology 2009 Jul 15;587(Pt 14):3639-50

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 66

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in a subset of reaction center cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.