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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00010411-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010411-D01, RRID:AB_10633400
- Product name
- RAPGEF3 MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human RAPGEF3 protein.
- Antigen sequence
MVLRRMHRPRSCSYQLLLEHQRPSCIQGLRWTPLT
NSEESLDFSESLEQASTERVLRAGRQLHRHLLATC
PNLIRDRKYHLRLYRQCCSGRELVDGILALGLGVH
SRSQVVGICQVLLDEGALCHVKHDWAFQDRDAQFY
RFPGPEPEPVGTHEMEEELAEAVALLSQRGPDALL
TVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNS
VKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIW
KGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRA
ATIILREDNCHFLRVDKQDFNRIIKDVEAKTMRLE
EHGKVVLVLERASQGAGPSRPPTPGRNRYTVMSGT
PEKILELLLEAMGPDSSAHDPTETFLSDFLLTHRV
FMPSAQLCAALLHHFHVEPAGGSEQERSTYVCNKR
QQILRLVSQWVALYGSMLHTDPVATSFLQKLSDLV
GRDTRLSNLLREQWPERRRCHRLENGCGNASPQMK
ARNLPVWLPNQDEPLPGSSCAIQVGDKVPYDICRP
DHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLV
KVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQE
VHELIPHPDQLGPTVGSAEGLDLVSAKDLAGQLTD
HDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERF
MRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLA
AHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLP
HKVRKLYSALERLLDPSWNHRVYRLALAKLSPPVI
PFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMAR
AARMLHHCRSHNPVPLSPLRSRVSHLHEDSQVARI
STCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRL
SRELEP- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of RAPGEF3 expression in transfected 293T cell line (H00010411-T02) by RAPGEF3 MaxPab polyclonal antibody.Lane 1: RAPGEF3 transfected lysate(99.40 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to RAPGEF3 on HeLa cell. [antibody concentration 30 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of RAPGEF3 transfected lysate using anti-RAPGEF3 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RAPGEF3 MaxPab rabbit polyclonal antibody (D01) (H00010411-D01).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
