NBP1-47733

antibody from Novus Biologicals

Targeting: ERCC1 RAD10

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-47733

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-47733, RRID:AB_10010700

Product name

Mouse Monoclonal ERCC1 Antibody

Antibody type

Monoclonal

Description

Affinity purified. This antibody is specific for Homo sapiens excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence) (ERCC1), transcript variant 2.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

0.55 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ERCC1 Antibody (1A3) [NBP1-47733] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ERCC1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ERCC1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ERCC1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded Ovary tissue using anti-ERCC1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded prostate tissue using anti-ERCC1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded thyroid tissue using anti-ERCC1 mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: ERCC1 Antibody (1A3) [NBP1-47733] - HEK293T cells transfected with either pCMV6-ENTRY ERCC1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-ERCC1 mouse monoclonal, and then analyzed by flow cytometry.