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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [4]
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- Product number
- PA5-40361 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fra1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: TDFLQAETDK LEDEKSGLQR EIEELQKQKE RLELVLEAHR PICKIPEGAK
- Concentration
- 0.5 mg/mL
Submitted references Epigenomic State Transitions Characterize Tumor Progression in Mouse Lung Adenocarcinoma.
Growth Plate Borderline Chondrocytes Behave as Transient Mesenchymal Precursor Cells.
LaFave LM, Kartha VK, Ma S, Meli K, Del Priore I, Lareau C, Naranjo S, Westcott PMK, Duarte FM, Sankar V, Chiang Z, Brack A, Law T, Hauck H, Okimoto A, Regev A, Buenrostro JD, Jacks T
Cancer cell 2020 Aug 10;38(2):212-228.e13
Cancer cell 2020 Aug 10;38(2):212-228.e13
Growth Plate Borderline Chondrocytes Behave as Transient Mesenchymal Precursor Cells.
Mizuhashi K, Nagata M, Matsushita Y, Ono W, Ono N
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2019 Aug;34(8):1387-1392
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2019 Aug;34(8):1387-1392
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat cell lysate using an anti-Fra-1 polyclonal antibody (Product # PA5-40361).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using anti-Fra1 Polyclonal Antibody (Product # PA5-40361) and a 39 kDa band corresponding to Fra1 was observed across cell lines tested along with an uncharacterized band (*) at ~70 kDa. Whole cell extracts (30 µg lysate) of U2-OS (Lane 1), A-375 (Lane 2), HeLa (Lane 3), K-562 (Lane 4), HEK-293 (Lane 5), Jurkat (Lane 6) and U-87MG (Lane 7) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human alveolar tissue using an anti-Fra-1 polyclonal antibody (Product # PA5-40361).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human alveolar tissue using an anti-Fra-1 polyclonal antibody (Product # PA5-40361).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human skin tissue using an anti-Fra-1 polyclonal antibody (Product # PA5-40361).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human skin tissue using an anti-Fra-1 polyclonal antibody (Product # PA5-40361).
