Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 100-4166 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-4166, RRID:AB_2179026
- Product name
- Anti-NFKB cRel (RABBIT) Antibody - 100-4166
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Targeting activation of specific NF-κB subunits prevents stress-dependent atherothrombotic gene expression.
Djuric Z, Kashif M, Fleming T, Muhammad S, Piel D, von Bauer R, Bea F, Herzig S, Zeier M, Pizzi M, Isermann B, Hecker M, Schwaninger M, Bierhaus A, Nawroth PP
Molecular medicine (Cambridge, Mass.) 2012 Dec 20;18(1):1375-86
Molecular medicine (Cambridge, Mass.) 2012 Dec 20;18(1):1375-86
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.
- Validation comment
- Western Blot