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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA308881 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal antibody to GAD67 (glutamate decarboxylase 1 (brain, 67kDa))
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal antibody to GAD67 (glutamate decarboxylase 1 (brain, 67kDa))
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- GAD1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Hippocampal GAD67 Transduction Using rAAV8 Regulates Epileptogenesis in EL Mice.
Shimazaki K, Kobari T, Oguro K, Yokota H, Kasahara Y, Murashima Y, Watanabe E, Kawai K, Okada T
Molecular therapy. Methods & clinical development 2019 Jun 14;13:180-186
Molecular therapy. Methods & clinical development 2019 Jun 14;13:180-186
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAD67 antibody detects GAD67 protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 7.5 % SDS-PAGE. GAD67 antibody (TA308881) dilution: 1:500
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAD67 antibody detects GAD67 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human GAD1-transfected 293T cells. 7.5 % SDS-PAGE. GAD67 antibody (TA308881) dilution: 1:5000
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAD67 antibody detects GAD67 protein by Western blot analysis. A. 30 ug SK-N-SH whole cell lysate/extract. B. 30 ug SK-N-AS whole cell lysate/extract. 7.5 % SDS-PAGE. GAD67 antibody (TA308881) dilution: 1:500
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAD67 antibody detects GAD67 protein by Western blot analysis. A. 50 ug mouse brian lysate/extract. 7.5 % SDS-PAGE. GAD67 antibody (TA308881) dilution: 1:500
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAD67 antibody detects GAD67 protein by immunofluorescent analysis. @Sample: GAD67-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAD67 antibody detects GAD67 protein at on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. GAD67 antibody (TA308881) dilution: 1:500.
- Validation comment
- IHC
