Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS4082 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS4082, RRID:AB_1664074
- Product name
- GSK3Ï« (phospho S21) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human GSK3Ï« around the phosphorylation site of Serine 21.
- Description
- Glycogen synthase kinase-3 Ï« and ÉÇ (GSK-3Ï«, ÉÇ) are serine/threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3ÉÇ activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFÏ« in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3Ï« and GSK-3ÉÇ induces phosphorylation of Ser 21 and Ser 9, respectively through a phosphatidylinositol 3-kinase (PI 3-kinase)-protein kinase B (PKB) dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3Ï« or Ser 9 of GSK-3ÉÇ and inactivates both forms
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-GSK3¦Á (S21) pAb at 1:500 dilutionLane1:HEK293T cell lysate treated with UVLane2:Mouse liver tissue lysateLane3:Rat liver tissue lysateLane4:PC12 cell lysate treated with H2O2