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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- bs-4692R - Provider product page
- Provider
- Bioss
- Proper citation
- Bioss Cat#bs-4692R, RRID:AB_11104259
- Product name
- GSK3 Alpha(Ser21) Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- KLH conjugated synthetic phosphopeptide derived from human GSK3 Alpha around the phosphorylation site of Ser21
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1ug/ul
- Storage
- Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Submitted references Effect of shRNA-mediated inhibition of Nanog gene expression on the behavior of human gastric cancer cells.
Ji W, Jiang Z
Oncology letters 2013 Aug;6(2):367-374
Oncology letters 2013 Aug;6(2):367-374
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Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti phospho-GSK3 Alpha(Ser21) Polyclonal Antibody, Unconjugated (bs-4692R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band 54kD. Observed band size: 54kD.
- Sample type
- Rat
- Other comments
- Heart
Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded rat brain labeled with Anti-phospho-GSK3 Alpha(Ser21) Polyclonal Antibody, Unconjugated (bs-4692R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
- Sample type
- Rat
- Other comments
- Brain
