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Learnsc-46672
antibody from Santa Cruz Biotechnology
Targeting: HDAC4
BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, KIAA0288
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-46672 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-46672, RRID:AB_627706
- Product name
- Anti-HDAC4
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references HDAC4 and PCAF bind to cardiac sarcomeres and play a role in regulating myofilament contractile activity.
Gupta MP, Samant SA, Smith SH, Shroff SG
The Journal of biological chemistry 2008 Apr 11;283(15):10135-46
The Journal of biological chemistry 2008 Apr 11;283(15):10135-46
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 119
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
