A00971-1

antibody from Boster Biological Technology

Targeting: HDAC4 BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, KIAA0288

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00971-1

Provider

Boster Biological Technology

Product name

Anti-HDAC4 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for Histone Deacetylase 4/HDAC4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, ICC/IF, FCM. Reactive species: Human;Mouse;Rat. Histone Deacetylase 4/HDAC4 information: Molecular Weight: 119040 MW; Subcellular Localization: Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation; Tissue Specificity: Ubiquitous.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of THP-1 cells using anti-HDAC4 antibody (A00971-1). Overlay histogram showing THP-1 cells stained with A00971-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- HDAC4 Antibody (A00971-1,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image