MAB6889

antibody from R&D Systems

Targeting: HOMER1 HOMER-1B, SYN47, Ves-1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6889

Provider

R&D Systems

Product name

Human/Mouse/Rat HOMER1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human HOMER1 in direct ELISAs.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q86YM7


Isotype

IgG

Antibody clone number

666241

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human HOMER1 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-HOMER1 Monoclonal Antibody (Catalog # MAB6889) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HOMER1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse HOMER1 by Simple Western^TM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for HOMER1 at approximately 51 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat HOMER1 Monoclonal Antibody (Catalog # MAB6889). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.