sc-28333

antibody from Santa Cruz Biotechnology

Targeting: HOXB13

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-28333

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-28333, RRID:AB_627744

Product name

Anti-HOXB13

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

References

Submitted references

Predictive relevance of HOXB13 protein expression for tamoxifen benefit in breast cancer.

Jerevall PL, Jansson A, Fornander T, Skoog L, Nordenskjöld B, Stål O
Breast cancer research : BCR 2010;12(4):R53

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Unique molecular characteristics of pediatric myxopapillary ependymoma.

Barton VN, Donson AM, Kleinschmidt-DeMasters BK, Birks DK, Handler MH, Foreman NK
Brain pathology (Zurich, Switzerland) 2010 May;20(3):560-70

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Epigenetic repression of the estrogen-regulated Homeobox B13 gene in breast cancer.

Rodriguez BA, Cheng AS, Yan PS, Potter D, Agosto-Perez FJ, Shapiro CL, Huang TH
Carcinogenesis 2008 Jul;29(7):1459-65

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 31

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.