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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-36812 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-IFNGR1 (Tyr457) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 54 and 90 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Phospho-IFNGR1 (Tyr457) in Lane 1: HEK293T cell lysate treated with IFNγ (100 µg/mL, 15 mins), Lane 2: HNIH-3T3 cell lysate treated with IFNγ (100 µg/mL, 15 mins), Lane 3: H9C2 cell lysate treated with IFNγ (100 µg/mL, 15 mins). Samples were incubated with Phospho-IFNGR1 (Tyr457) polyclonal antibody (Product # PA5-36812) at a dilution of 1:500.
