MAB6731

antibody from Novus Biologicals

Targeting: IFNGR1 CD119, IFNGR

Western blot (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6731

Provider

Novus Biologicals

Product name

Mouse Monoclonal IFN-gamma R1/CD119 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human IFN-gamma R1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse IFN-gamma R1, recombinant human (rh) IFN-gamma R2, or rhIL-10 R beta .

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

500 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Single-cell transcriptomics of human T cells reveals tissue and activation signatures in health and disease.

Szabo PA, Levitin HM, Miron M, Snyder ME, Senda T, Yuan J, Cheng YL, Bush EC, Dogra P, Thapa P, Farber DL, Sims PA
Nature communications 2019 Oct 17;10(1):4706

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Ankyrin repeat domain 1 regulates innate immune responses against herpes simplex virus 1: A potential role in eczema herpeticum.

Bin L, Li X, Richers B, Streib JE, Hu JW, Taylor P, Leung DYM
The Journal of allergy and clinical immunology 2018 Jun;141(6):2085-2093.e1

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Casein Kinase 1α Mediates the Degradation of Receptors for Type I and Type II Interferons Caused by Hemagglutinin of Influenza A Virus.

Xia C, Wolf JJ, Vijayan M, Studstill CJ, Ma W, Hahm B
Journal of virology 2018 Apr 1;92(7)

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Bispecific T cell engager (BiTE®) antibody constructs can mediate bystander tumor cell killing.

Ross SL, Sherman M, McElroy PL, Lofgren JA, Moody G, Baeuerle PA, Coxon A, Arvedson T
PloS one 2017;12(8):e0183390

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IL-1β induced HIF-1α inhibits the differentiation of human FOXP3⁺ T cells.

Feldhoff LM, Rueda CM, Moreno-Fernandez ME, Sauer J, Jackson CM, Chougnet CA, Rupp J
Scientific reports 2017 Mar 28;7(1):465

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Induction of indoleamine 2,3-dioxygenase by Borrelia burgdorferi in human immune cells correlates with pathogenic potential.

Love AC, Schwartz I, Petzke MM
Journal of leukocyte biology 2015 Feb;97(2):379-90

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Interferon-gamma differentially regulates TGF-beta1 and TGF-beta2 expression in human retinal pigment epithelial cells through JAK-STAT pathway.

Nagineni CN, Cherukuri KS, Kutty V, Detrick B, Hooks JJ
Journal of cellular physiology 2007 Jan;210(1):192-200

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Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.

Ebert EC, Mehta V
Cellular immunology 2006 May;241(1):7-13

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-gamma R1/CD119 Antibody. Recombinant Human IFN-gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Anti-Human IFN-gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Human IFN-gamma R1/CD119 Monoclonal Antibody (Catalog # MAB6731). The ND₅₀ is typically 0.01-0.03 µg/mL.