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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- 10808-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFNGR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: TQKEDDCDE IQCQLAIPVS SLNSQYCVSA EGVLHVWGVT TEKSKEVCIT IFNSSIKGSL WIPVVAALLL FLVLSLVFIC FYIKKINPLK EKSIILPKSL ISVVRSATLE TKPESKYVSL ITSYQPFSLE KEVVCEEPLS PATVPGMHTE DNPGKVEHTE ELSSITEVVT TEENIPDVVP GSHLTPIERE SSSPLSSNQS EPGSIALNSY HSRNCSESDH SRNGFDTDSS CLESHSSLSD SEFPPNNKGE IKTEGQELIT VIKAPTSFGY DKPHVLVDLL VDDSGKESLI GYRPTEDSKE FS (189-489 aa encoded by BC005333)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.53 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10808-1-AP ( IFNGR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 10808-1-AP (IFNGR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 10808-1-AP ( IFNGR1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 10808-1-AP ( IFNGR1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with .2ug IFNGR1 antibody (10808-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-IFNGR1 (IP:10808-1-AP, 4ug; Detection:10808-1-AP 1:500) with HepG2 cells lysate 1800ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-IFNGR1 (IP:10808-1-AP, 4 ug; Detection:10808-1-AP 1:300) with HepG2 cells lysate 3000 ug.
