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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX129006 - Provider product page
- Provider
- GeneTex
- Product name
- IGFBP1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IGFBP1 antibody detects IGFBP1 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP1 antibody (GTX129006) diluted by 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP1 antibody (GTX129006) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IGFBP1 antibody detects IGFBP1 protein at cytoplasm and Golgi apparatus by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IGFBP1 stained by IGFBP1 antibody (GTX129006) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of IGFBP1 protein from HepG2 whole cell extracts using 5 £gg of IGFBP1 antibody (GTX129006).Western blot analysis was performed using IGFBP1 antibody (GTX129006).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
