sc-9983

antibody from Santa Cruz Biotechnology

Targeting: IL1A IL-1A, IL1, IL1-ALPHA, IL1F1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-9983

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-9983, RRID:AB_627789

Product name

Anti-IL1A

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

References

Submitted references

Epithelial Cells Promote Fibroblast Activation via IL-1α in Systemic Sclerosis

Nima Aden, Anna Nuttall, Xu Shiwen, Patricia de Winter, Andrew Leask, Carol M Black, Christopher P Denton, David J Abraham, Richard J Stratton
Journal of Investigative Dermatology 2010 May;130(9):2191-2200

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 31

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.