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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-400-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-1 alpha/IL-1F1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IL-1 alpha/IL-1F1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant rat IL-1 alpha and recombinant cotton rat IL-1 alpha is observed and less than 1% cross-reactivity with recombinant human IL-1 alpha is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Ptpn6 inhibits caspase-8- and Ripk3/Mlkl-dependent inflammation.
Tacrolimus interacts with voriconazole to reduce the severity of fungal keratitis by suppressing IFN-related inflammatory responses and concomitant FK506 and voriconazole treatment suppresses fungal keratitis.
TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects.
Interleukin-1 and estrogen protect against disseminating dentoalveolar infections.
AIM2 and NLRC4 inflammasomes contribute with ASC to acute brain injury independently of NLRP3.
IL-1α signaling is critical for leukocyte recruitment after pulmonary Aspergillus fumigatus challenge.
The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines.
Dendritic cell IL-1α and IL-1β are polyubiquitinated and degraded by the proteasome.
Interleukin-1 deficiency prolongs ovarian lifespan in mice.
IL-1α and IL-1β recruit different myeloid cells and promote different stages of sterile inflammation.
Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1β in type 2 diabetes.
Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening.
Speir M, Nowell CJ, Chen AA, O'Donnell JA, Shamie IS, Lakin PR, D'Cruz AA, Braun RO, Babon JJ, Lewis RS, Bliss-Moreau M, Shlomovitz I, Wang S, Cengia LH, Stoica AI, Hakem R, Kelliher MA, O'Reilly LA, Patsiouras H, Lawlor KE, Weller E, Lewis NE, Roberts AW, Gerlic M, Croker BA
Nature immunology 2020 Jan;21(1):54-64
Nature immunology 2020 Jan;21(1):54-64
Tacrolimus interacts with voriconazole to reduce the severity of fungal keratitis by suppressing IFN-related inflammatory responses and concomitant FK506 and voriconazole treatment suppresses fungal keratitis.
Zhong J, Peng L, Wang B, Zhang H, Li S, Yang R, Deng Y, Huang H, Yuan J
Molecular vision 2018;24:187-200
Molecular vision 2018;24:187-200
TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects.
Choo MK, Sano Y, Kim C, Yasuda K, Li XD, Lin X, Stenzel-Poore M, Alexopoulou L, Ghosh S, Latz E, Rifkin IR, Chen ZJ, Stewart GC, Chong H, Park JM
The Journal of experimental medicine 2017 May 1;214(5):1297-1311
The Journal of experimental medicine 2017 May 1;214(5):1297-1311
Interleukin-1 and estrogen protect against disseminating dentoalveolar infections.
Youssef H, Stashenko P
International journal of oral science 2017 Mar;9(1):16-23
International journal of oral science 2017 Mar;9(1):16-23
AIM2 and NLRC4 inflammasomes contribute with ASC to acute brain injury independently of NLRP3.
Denes A, Coutts G, Lénárt N, Cruickshank SM, Pelegrin P, Skinner J, Rothwell N, Allan SM, Brough D
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 31;112(13):4050-5
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 31;112(13):4050-5
IL-1α signaling is critical for leukocyte recruitment after pulmonary Aspergillus fumigatus challenge.
Caffrey AK, Lehmann MM, Zickovich JM, Espinosa V, Shepardson KM, Watschke CP, Hilmer KM, Thammahong A, Barker BM, Rivera A, Cramer RA, Obar JJ
PLoS pathogens 2015 Jan;11(1):e1004625
PLoS pathogens 2015 Jan;11(1):e1004625
The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines.
Asrat S, Dugan AS, Isberg RR
PLoS pathogens 2014 Jul;10(7):e1004229
PLoS pathogens 2014 Jul;10(7):e1004229
Dendritic cell IL-1α and IL-1β are polyubiquitinated and degraded by the proteasome.
Ainscough JS, Frank Gerberick G, Zahedi-Nejad M, Lopez-Castejon G, Brough D, Kimber I, Dearman RJ
The Journal of biological chemistry 2014 Dec 19;289(51):35582-92
The Journal of biological chemistry 2014 Dec 19;289(51):35582-92
Interleukin-1 deficiency prolongs ovarian lifespan in mice.
Uri-Belapolsky S, Shaish A, Eliyahu E, Grossman H, Levi M, Chuderland D, Ninio-Many L, Hasky N, Shashar D, Almog T, Kandel-Kfir M, Harats D, Shalgi R, Kamari Y
Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 26;111(34):12492-7
Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 26;111(34):12492-7
IL-1α and IL-1β recruit different myeloid cells and promote different stages of sterile inflammation.
Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 1;187(9):4835-43
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 1;187(9):4835-43
Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1β in type 2 diabetes.
Masters SL, Dunne A, Subramanian SL, Hull RL, Tannahill GM, Sharp FA, Becker C, Franchi L, Yoshihara E, Chen Z, Mullooly N, Mielke LA, Harris J, Coll RC, Mills KH, Mok KH, Newsholme P, Nuñez G, Yodoi J, Kahn SE, Lavelle EC, O'Neill LA
Nature immunology 2010 Oct;11(10):897-904
Nature immunology 2010 Oct;11(10):897-904
Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening.
Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM
Bone 2007 May;40(5):1276-83
Bone 2007 May;40(5):1276-83
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Recombinant Mouse and Rat IL-1 alpha/IL-1F1 by Western Blot. Western blot shows 50 ng of Recombinant Mouse IL-1 alpha/IL-1F1 (Catalog # 400-ML), Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA) and Recombinant Rat IL-1 alpha/IL-1F1 (Catalog # 500-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL-1 alpha/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-1 alpha/IL-1F1 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-1 alpha/IL-1F1 in Mouse Liver. IL-1 alpha/IL-1F1 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse IL-1 alpha/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-1 alpha/IL-1F1 and Neutral-ization by Mouse IL-1 alpha/IL-1F1 Antibody. Recombinant Mouse IL-1 alpha/IL-1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 alpha/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 alpha/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA). The ND50 is typically 0.002-0.004 µg/mL in the presence of concanavalin A (1.25 µg/mL).
