H00003552-M04

antibody from Abnova Corporation

Targeting: IL1A IL-1A, IL1, IL1-ALPHA, IL1F1

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003552-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003552-M04, RRID:AB_10554180

Product name

IL1A monoclonal antibody (M04), clone 4F9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant IL1A.

Antigen sequence

KSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKE
MPEIPKTITGSETNLLFFWETHGTKNYFTSVAHPN
LFIATKQDYWVCLAGGPPSITDFQILENQA


Isotype

IgG

Antibody clone number

4F9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged IL1A is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to IL1A on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of IL1A transfected lysate using anti-IL1A monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IL1A MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol