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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AM20905PU-N - Provider product page
- Provider
- OriGene
- Product name
- IRAK (IRAK1) mouse monoclonal antibody, clone 3A9
- Antibody type
- Monoclonal
- Description
- IRAK (IRAK1) mouse monoclonal antibody, clone 3A9
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- IRAK1
- Isotype
- IgG
- Antibody clone number
- 3A9
- Vial size
- 50 µg
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody clone 3A9.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IRAK1 monoclonal antibody clone 3A9 Western Blot analysis of IRAK1 expression in A-431.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB analysis of IRAK1 over-expressed 293 cell line, cotransfected with IRAK1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with IRAK1 MAb clone 3A9. GAPDH ( 36.1 kD ) used as specificity and loading ctrl.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged IRAK1 is approximately 0.03 ng/ml as a capture antibody.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to IRAK1 on HeLa cell (antibody concentration 10 ug/ml).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Validation comment
- IHC
