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LearnH00003654-M02
antibody from Novus Biologicals
Targeting: IRAK1
IRAK, pelle
Western blot ELISA Immunocytochemistry Immunoprecipitation Immunohistochemistry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00003654-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003654-M02, RRID:AB_539905
- Product name
- Mouse Monoclonal IRAK1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. IRAK1 - interleukin-1 receptor-associated kinase 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of IRAK1 over-expressed 293 cell line, cotransfected with IRAK1 Validated Chimera RNAi ( Cat # H00003654-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRAK1 monoclonal antibody (M02) clone 3A9 (Cat # H00003654-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody (M02), clone 3A9.Lane 1: IRAK1 transfected lysate(68 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRAK1 Antibody (3A9) [H00003654-M02] - IRAK1 monoclonal antibody (M02), clone 3A9 Analysis of IRAK1 expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: IRAK1 Antibody (3A9) [H00003654-M02] - Detection limit for recombinant GST tagged IRAK1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of monoclonal antibody to IRAK1 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of IRAK1 transfected lysate using anti-IRAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRAK1 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of monoclonal antibody to IRAK1 on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.
