Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-44983 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRKCSH Monoclonal Antibody (A6H9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- A6H9
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Glucosidase 2 subunit beta in different lysates (10 µg/Lane). Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in Glucosidase 2 subunit beta Monoclonal antibody (Product # MA5-44983) using a dilution of 1:500 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Mouse IgG - HRP secondary antibody at a dilution of 1:20,000 for 1 hour at room temperature. Lane 1: Hela cell lysate; Lane 2: 293T cell lysate. Predicted band size: 59 kDa. Observed band size: 77 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Glucosidase 2 subunit beta in HeLa cells. Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated in Glucosidase 2 subunit beta Monoclonal antibody (Product # MA5-44983) using a dilution of 1:100 in 1% BSA overnight at 4 ℃ followed by Goat Anti-Mouse IgG H&L (iFluor™ 488) secondary antibody at a dilution of 1:1,000. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Glucosidase 2 subunit beta in paraffin-embedded human placenta tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with Glucosidase 2 subunit beta Monoclonal antibody (Product # MA5-44983) using a dilution of 1:200 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Glucosidase 2 subunit beta in HeLa cells. Cells were fixed and permeabilized then stained with Glucosidase 2 subunit beta Monoclonal antibody (Product # MA5-44983) using a dilution of 1 µg/mL (red) at 4℃ for an hour followed by iFluor™ 488 conjugate-Goat anti-Mouse IgG secondary antibody at a dilution of 1:1,000 for 30 minutes at 4℃. Mouse IgG1 Isotype Control (green). Unlabeled sample was used as a control (cells without incubation with primary antibody; black).