MA5-44983

antibody from Invitrogen Antibodies

Targeting: PRKCSH G19P1, GIIB, PCLD, PLD1, VASAP-60

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-44983

Provider

Invitrogen Antibodies

Product name

PRKCSH Monoclonal Antibody (A6H9)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

A6H9

Vial size

100 µL

Concentration

2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Glucosidase 2 subunit beta in different lysates (10 µg/Lane). Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in Glucosidase 2 subunit beta Monoclonal antibody (Product # MA5-44983) using a dilution of 1:500 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Mouse IgG - HRP secondary antibody at a dilution of 1:20,000 for 1 hour at room temperature. Lane 1: Hela cell lysate; Lane 2: 293T cell lysate. Predicted band size: 59 kDa. Observed band size: 77 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Glucosidase 2 subunit beta in HeLa cells. Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated in Glucosidase 2 subunit beta Monoclonal antibody (Product # MA5-44983) using a dilution of 1:100 in 1% BSA overnight at 4 ℃ followed by Goat Anti-Mouse IgG H&L (iFluor™ 488) secondary antibody at a dilution of 1:1,000. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Glucosidase 2 subunit beta in paraffin-embedded human placenta tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with Glucosidase 2 subunit beta Monoclonal antibody (Product # MA5-44983) using a dilution of 1:200 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Glucosidase 2 subunit beta in HeLa cells. Cells were fixed and permeabilized then stained with Glucosidase 2 subunit beta Monoclonal antibody (Product # MA5-44983) using a dilution of 1 µg/mL (red) at 4℃ for an hour followed by iFluor™ 488 conjugate-Goat anti-Mouse IgG secondary antibody at a dilution of 1:1,000 for 30 minutes at 4℃. Mouse IgG1 Isotype Control (green). Unlabeled sample was used as a control (cells without incubation with primary antibody; black).