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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27551 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRKCSH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A549, HeLa.
- Concentration
- 0.85 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PRKCSH Polyclonal Antibody (Product # PA5-27551). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: HeLa . 7.5% SDS PAGE. PRKCSH Polyclonal Antibody (Product # PA5-27551) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRKCSH Polyclonal Antibody detects PRKCSH protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRKCSH protein stained by PRKCSH Polyclonal Antibody (Product # PA5-27551) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRKCSH Polyclonal Antibody detects PRKCSH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. PRKCSH Polyclonal Antibody (Product # PA5-27551) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
