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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00007520-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007520-M02, RRID:AB_463926
- Product name
- XRCC5 monoclonal antibody (M02), clone 3D8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant XRCC5.
- Antigen sequence
MVRSGNKAAVVLCMDVGFTMSNSIPGIESPFEQAK
KVITMFVQRQVFAENKDEIALVLFGTDGTDNPLSG
GDQYQNITVHRHLMLPDFDLLEDIESKIQPGSQQA
DSLDALIVSMDVIQHETIGKKFEKRHIEIFTDLSS
RFSKSQLDIIIHSLKKCDISLQFFLPFSLGKEDGS
GDRGDGPFRLGGHGPSFPLKGITEQQKEGLEIVKM
VMISLEGEDGLDEIYSFSESLRKLCVFKKIERHSI
HWPCRLTIGSNLSIRIAAYKSILQERVKKTWTVVD
AKTLKKEDIQKETVYCLNDDDETEVLKEDIIQGFR
YGSDIVPFSKVDEEQMKYKSEGKCFSVLGFCKSSQ
VQRRFFMGNQVLKVFAARDDEAAAVALSSLIHALD
DLDMVAIVRYAYDKRANPQVGVAFPHIKHNYECLV
YVQLPFMEDLRQYMFSSLKNSKKYAPTEAQLNAVD
ALIDSMSLAKKDEKTDTLEDLFPTTKIPNPRFQRL
FQCLLHRALHPREPLPPIQQHIWNMLNPPAEVTTK
SQIPLSKIKTLFPLIEAKKKDQVTAQEIFQDNHED
GPTAKKLKTEQGGAHFSVSSLAEGSVTSVGSVNPA
ENFRVLVKQKKASFEEASNQLINHIEQFLDTNETP
YFMKSIDCIRAFREEAIKFSEEQRFNNFLKALQEK
VEIKQLNHFWEIVVQDGIALITKEEASGSSVTAEE
AKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI- Isotype
- IgG
- Antibody clone number
- 3D8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Serum anti-Ku86 is a potential biomarker for early detection of hepatitis C virus-related hepatocellular carcinoma.
Nomura F, Sogawa K, Noda K, Seimiya M, Matsushita K, Miura T, Tomonaga T, Yoshitomi H, Imazeki F, Takizawa H, Mogushi K, Miyazaki M, Yokosuka O
Biochemical and biophysical research communications 2012 May 18;421(4):837-43
Biochemical and biophysical research communications 2012 May 18;421(4):837-43
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- XRCC5 monoclonal antibody (M02), clone 3D8. Western Blot analysis of XRCC5 expression in A-431 ( Cat # L015V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of XRCC5 expression in transfected 293T cell line by XRCC5 monoclonal antibody (M02), clone 3D8.Lane 1: XRCC5 transfected lysate(82.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged XRCC5 is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to XRCC5 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to XRCC5 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
