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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- ELISA [1]
- Flow cytometry [2]
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- Product number
- ABIN108718 - Provider product page
- Provider
- antibodies-online
- Proper citation
- Antibodies-Online Cat#ABIN108718, RRID:AB_10953911
- Product name
- anti-Interleukin 13 Receptor, alpha 1 (IL13RA1) (Extracellular Domain) antibody
- Antibody type
- Monoclonal
- Antigen
- genetic immunisation with cDNA encoding human IL13-Ralpha1 (extracellular domain)
- Description
- Protein G
- Reactivity
- Human
- Epitope
- Extracellular Domain
- Isotype
- IgG
- Antibody clone number
- GM-1E7
- Vial size
- 100 μg
- Storage
- short term: 2°C - 8°C, long term: -20°C
- Handling
- Avoid repeated freezing and thawing.
Submitted references Targeting the IL-4/IL-13 signaling pathway sensitizes Hodgkin lymphoma cells to chemotherapeutic drugs.
Blockade of interleukin-13-mediated cell activation by a novel inhibitory antibody to human IL-13 receptor alpha1.
Expression of interleukin-13 receptor alpha 1-subunit on peripheral blood eosinophils is regulated by cytokines.
Pro-oxidant effects of normobaric hyperoxia in rat tissues.
Natoli A, Lüpertz R, Merz C, Müller WW, Köhler R, Krammer PH, Li-Weber M
International journal of cancer. Journal international du cancer 2013 Oct 15;133(8):1945-54
International journal of cancer. Journal international du cancer 2013 Oct 15;133(8):1945-54
Blockade of interleukin-13-mediated cell activation by a novel inhibitory antibody to human IL-13 receptor alpha1.
Krause S, Behrends J, Borowski A, Lohrmann J, Lang S, Myrtek D, Lorenzen T, Virchow JC, Luttmann W, Friedrich K
Molecular immunology 2006 Apr;43(11):1799-807
Molecular immunology 2006 Apr;43(11):1799-807
Expression of interleukin-13 receptor alpha 1-subunit on peripheral blood eosinophils is regulated by cytokines.
Myrtek D, Knoll M, Matthiesen T, Krause S, Lohrmann J, Schillinger D, Idzko M, Virchow JC, Friedrich K, Luttmann W
Immunology 2004 Aug;112(4):597-604
Immunology 2004 Aug;112(4):597-604
Pro-oxidant effects of normobaric hyperoxia in rat tissues.
Ahotupa M, Mäntylä E, Peltola V, Puntala A, Toivonen H
Acta physiologica Scandinavica 1992 Jun;145(2):151-7
Acta physiologica Scandinavica 1992 Jun;145(2):151-7
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified E7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified E7 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Dose-dependent inhibition of cellular proliferation. Samples of TF-1 cells were incubated for 72 h with 10 nM human IL-13 (closed circles) or 10 nM human IL-4 (open circles) plus the indicated concentrations of purified E7. Antibody GM1E7 blocks IL-13-dependent cell proliferation completely,
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- FACS analysis of BOSC23 cells using E6. BOSC23 cells were transiently transfected with an expression vector encoding either IL13-R (red curve) or an irrelevant protein (control transfectant: black curve). Binding of E7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with IL13-R transfected cells.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- FACS analysis of BOSC23 cells using E6. BOSC23 cells were transiently transfected with an expression vector encoding either IL13-R (red curve) or an irrelevant protein (control transfectant: black curve). Binding of E7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with IL13-R transfected cells.
