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- Antibody Data
- Antigen structure
- References [4]
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- Western blot [1]
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- Product number
- AF1505 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LRP-6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human LRP-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse LRP-6 is observed, and less than 1% cross-reactivity with recombinant human (rh) LRP-1, rhLRP-4, and rhLRP-5 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O75581- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A novel high-content imaging-based technique for measuring binding of Dickkopf-1 to low-density lipoprotein receptor-related protein 6.
The Novel Secreted Adipokine WNT1-inducible Signaling Pathway Protein 2 (WISP2) Is a Mesenchymal Cell Activator of Canonical WNT.
Activation of canonical wingless-type MMTV integration site family (Wnt) signaling in mature adipocytes increases beta-catenin levels and leads to cell dedifferentiation and insulin resistance.
Cooperative folding and ligand-binding properties of LRP6 beta-propeller domains.
Priestley RS, Cheung J, Murphy EJ, Ehebauer MT, Davis JB, Di Daniel E
Journal of pharmacological and toxicological methods 2019 Jan - Feb;95:47-55
Journal of pharmacological and toxicological methods 2019 Jan - Feb;95:47-55
The Novel Secreted Adipokine WNT1-inducible Signaling Pathway Protein 2 (WISP2) Is a Mesenchymal Cell Activator of Canonical WNT.
Grünberg JR, Hammarstedt A, Hedjazifar S, Smith U
The Journal of biological chemistry 2014 Mar 7;289(10):6899-6907
The Journal of biological chemistry 2014 Mar 7;289(10):6899-6907
Activation of canonical wingless-type MMTV integration site family (Wnt) signaling in mature adipocytes increases beta-catenin levels and leads to cell dedifferentiation and insulin resistance.
Gustafson B, Smith U
The Journal of biological chemistry 2010 Apr 30;285(18):14031-41
The Journal of biological chemistry 2010 Apr 30;285(18):14031-41
Cooperative folding and ligand-binding properties of LRP6 beta-propeller domains.
Liu CC, Pearson C, Bu G
The Journal of biological chemistry 2009 May 29;284(22):15299-307
The Journal of biological chemistry 2009 May 29;284(22):15299-307
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human LRP-6 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected or transfected with human LRP-6. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human LRP-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1505) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for LRP-6 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
