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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB1505 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LRP-6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human LRP-6 in direct ELISAs. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) LRP-6 and no cross-reactivity with recombinant human (rh) LRP-1, -4, -5, rhLRP-6 (intracellular domain), or rhLDL R is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O75581- Isotype
- IgG
- Antibody clone number
- 255302
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references USP6 oncogene promotes Wnt signaling by deubiquitylating Frizzleds.
ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner.
Madan B, Walker MP, Young R, Quick L, Orgel KA, Ryan M, Gupta P, Henrich IC, Ferrer M, Marine S, Roberts BS, Arthur WT, Berndt JD, Oliveira AM, Moon RT, Virshup DM, Chou MM, Major MB
Proceedings of the National Academy of Sciences of the United States of America 2016 May 24;113(21):E2945-54
Proceedings of the National Academy of Sciences of the United States of America 2016 May 24;113(21):E2945-54
ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner.
Hao HX, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, Lei H, Mickanin C, Liu D, Ruffner H, Mao X, Ma Q, Zamponi R, Bouwmeester T, Finan PM, Kirschner MW, Porter JA, Serluca FC, Cong F
Nature 2012 Apr 29;485(7397):195-200
Nature 2012 Apr 29;485(7397):195-200
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LRP-6 in DU145 Human Cell Line. LRP-6 was detected in immersion fixed DU145 human prostate carcinoma cell line using Mouse Anti-Human LRP-6 Monoclonal Antibody (Catalog # MAB1505) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of LRP-6 in MDA- MB-231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human LRP-6 Monoclonal Antibody (Catalog # MAB1505, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
