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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-96440 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HELLS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VYRLVTANTI DQKIVERAAA KRKLEKLIIH KNHFKGGQSG LNLSKNFLDP KELMELLKSR DYEREIKGSR EKVISDKDLE LLLDRSDLID QMNASGPIKE KMGIFKILEN SEDSSPECLF; Positive Samples: MCF-7, A549, 293T, HepG2, Mouse thymus; Cellular Location: Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.51 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HELLS in extracts from normal (control) and HELLS knockout (KO) HeLa cells using HELLS Polyclonal Antibody (Product # PA5-96440) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HELLS in extracts of various cell lines using HELLS Polyclonal Antibody (Product # PA5-96440) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HELLS in paraffin-embedded mouse testis using HELLS Polyclonal Antibody (Product # PA5-96440) at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of HELLS was performed in 200 µg extracts of 293T cells using HELLS Polyclonal Antibody (Product # PA5-96440). Western blot was performed from the immunoprecipitate using HELLS Polyclonal Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of HELLS was performed in 200 µg extracts of 293T cells using HELLS Polyclonal Antibody (Product # PA5-96440). Western blot was performed from the immunoprecipitate using HELLS Polyclonal Antibody.
