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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- 11955-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HELLS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MFGSSEKET IELSPTGRPK RRTRKSINYS KIDDFPNELE KLISQIQPEV DRERAVVEVN IPVESEVNLK LQNIMMLLRK CCNHPYLIEY PIDPVTQEFK IDEELVTNSG KFLILDRMLP ELKKRGHKVL LFSQMTSMLD ILMDYCHLRD FNFSRLDGSM SYSEREKNMH SFNTDPEVFI FLVSTRAGGL GINLTAADTV IIYDSDWNPQ SDLQAQDRCH RIGQTKPVVV YRLVTANTID QKIVERAAAK RKLEKLIIHK NHFKGGQSGL NLSKNFLDPK ELMELLKSRD YEREIKGSRE KVISDKDLEL LLDRSDLIDQ MNASGPIKEK MGIFKILENS EDSSPECLF (1-348 aa encoded by BC031004 )
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DU 145 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using HELLS antibody 11955-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-HELLS (IP:11955-1-AP, 4ug; Detection:11955-1-AP 1:1000) with mouse testis tissue lysate 4000ug.
