PB9275

antibody from Boster Biological Technology

Targeting: ABCB4 GBD1, MDR2, MDR3, PFIC-3, PGY3

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PB9275

Provider

Boster Biological Technology

Product name

Anti-ABCB4 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for MDR3/ABCB4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: Flow Cytometry. Reactive species: Human. MDR3/ABCB4 information: Molecular Weight: 141523 MW; Subcellular Localization: Cell membrane; Multi-pass membrane protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of HEPG2 cells using anti-ABCB4 antibody (PB9275). Overlay histogram showing HEPG2 cells stained with PB9275 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCB4 Antibody (PB9275,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image