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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- LS-B67 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B67, RRID:AB_2250999
- Product name
- IHC-plus™ MYL12A / MRCL3 Antibody (phospho-Ser19) LS-B67
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Store at -20°C. Store undiluted.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-Myosin pS19/pS20 Antibody - Western Blot. Affinity Purified Phospho specific antibody to Monophosphorylated Regulatory Light Chain of Smooth and Non-muscle Myosin at pS19/pS20 was used at a 1:5000 dilution to detect myosin light chain by Western blot. Either 13 or 20 ul of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (LS-C60865) preceded color development using Amershams substrate system. Other detection methods will yield similar results. Data courtesy of the Alliance for Cellular Signaling (http://www. signaling-gateway. org).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-Myosin pS19/pS20 Antibody - Western Blot. Affinity purified phosphospecific antibody to phosphorylated regulatory light chain of smooth and non-muscle Myosin at pS19/pS20 was used at a 1:1000 dilution to detect myosin light chain by Western blot on 3T3 cell lysates. A standard urea/glycerol gel without SDS was used to separate phospho forms of regulatory light chain according to mass to charge ratios. In Panel A, reactivity of phosphospecific antibody is shown. In Panel B, reactivity of commercially available pan reactive antibody that detects both unphosphorylated and phosphorylated forms of regulatory light chain is shown. phosphospecific antibody detects both monophosphorylated (pSer20 Mono-P-RLC) and diphosphorylated (pThr19-pSer20 Di-P-RLC) regulatory light chain. Personal communication. J. Stull. UT Southwestern Medical Center.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-MYL9 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
