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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- TA319238 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal Myosin phospho S19/phospho S20 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal Myosin phospho S19/phospho S20 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MYL12A
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.92 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Phospho specific antibody to Monophosphorylated Regulatory Light Chain of Smooth and Non-muscle Myosin at pS19/pS20 was used at a 1:5000 dilution to detect myosin light chain by WB. Either 13 or 20 ?l of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. ?Samples were either mock-treated or CLA-treated, as indicated.? After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Phosphospecific antibody to phosphorylated regulatory light chain of smooth and non-muscle Myosin at pS19/pS20 was used at a 1:1000 dilution to detect myosin light chain by WB on 3T3 cell lysates.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-Monophosphorylated RLC Smooth and Non-Muscle Myosin pS19/20 antibody was used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
- Validation comment
- IHC
